Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo

In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced. Due to the bias in reverse transcription and PCR amplification, we could n...

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Detalles Bibliográficos
Autores principales: N, Monford Paul Abishek, Jeon, Heungjin, Wang, Xun, Lim, Heon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670590/
https://www.ncbi.nlm.nih.gov/pubmed/37998331
http://dx.doi.org/10.3390/cells12222596

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670590/
https://www.ncbi.nlm.nih.gov/pubmed/37998331
http://dx.doi.org/10.3390/cells12222596

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