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The Thermodynamic Fingerprints of Ultra-Tight Nanobody–Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection

Life on the molecular scale is based on a versatile interplay of biomolecules, a feature that is relevant for the formation of macromolecular complexes. Fluorescence-based two-color coincidence detection is widely used to characterize molecular binding and was recently improved by a brightness-gated...

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Detalles Bibliográficos
Autores principales: Schedler, Benno, Yukhnovets, Olessya, Lindner, Lennart, Meyer, Alida, Fitter, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671529/
https://www.ncbi.nlm.nih.gov/pubmed/38003569
http://dx.doi.org/10.3390/ijms242216379