Improvement of the Genome Editing Tools Based on 5FC/5FU Counter Selection in Clostridium acetobutylicum

Several genetic tools have been developed for genome engineering in Clostridium acetobutylicum utilizing 5-fluorouracil (5FU) or 5-fluorocytosine (5FC) resistance as a selection method. In our group, a method based on the integration, by single crossing over, of a suicide plasmid (pCat-upp) followed...

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Detalles Bibliográficos
Autores principales: Boudignon, Eglantine, Foulquier, Céline, Soucaille, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672894/
https://www.ncbi.nlm.nih.gov/pubmed/38004708
http://dx.doi.org/10.3390/microorganisms11112696

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672894/
https://www.ncbi.nlm.nih.gov/pubmed/38004708
http://dx.doi.org/10.3390/microorganisms11112696

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