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Improvement of the Genome Editing Tools Based on 5FC/5FU Counter Selection in Clostridium acetobutylicum

Several genetic tools have been developed for genome engineering in Clostridium acetobutylicum utilizing 5-fluorouracil (5FU) or 5-fluorocytosine (5FC) resistance as a selection method. In our group, a method based on the integration, by single crossing over, of a suicide plasmid (pCat-upp) followed...

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Detalles Bibliográficos
Autores principales:	Boudignon, Eglantine, Foulquier, Céline, Soucaille, Philippe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10672894/ https://www.ncbi.nlm.nih.gov/pubmed/38004708 http://dx.doi.org/10.3390/microorganisms11112696

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