Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair

BACKGROUND: Gene knock‐in (KI) in animal cells via homology‐directed repair (HDR) is an inefficient process, requiring a laborious work for screening from few modified cells. HDR tends to occur in the S and G2/M phases of cell cycle; therefore, strategies that enhance the proportion of cells in thes...

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Detalles Bibliográficos
Autores principales: Li, Guoling, Yang, Xiaohui, Luo, Xinxin, Wu, Zhenfang, Yang, Huaqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676593/
https://www.ncbi.nlm.nih.gov/pubmed/38007480
http://dx.doi.org/10.1186/s13578-023-01159-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676593/
https://www.ncbi.nlm.nih.gov/pubmed/38007480
http://dx.doi.org/10.1186/s13578-023-01159-4

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