Natural killer cell-related prognosis signature predicts immune response in colon cancer patients

Background: Natural killer (NK) cells are crucial components of the innate immune system that fight tumors and viral infections. Patients with colorectal cancer (CRC) have a poor prognosis, and immunotherapeutic tools play a key role in the treatment of CRC. Methods: Public data on CRC patients was...

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Autores principales: Li, Meiqin, Song, Jingqing, Wang, Lin, Wang, Qi, Huang, Qinghua, Mo, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679416/
https://www.ncbi.nlm.nih.gov/pubmed/38026928
http://dx.doi.org/10.3389/fphar.2023.1253169
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author Li, Meiqin
Song, Jingqing
Wang, Lin
Wang, Qi
Huang, Qinghua
Mo, Dan
author_facet Li, Meiqin
Song, Jingqing
Wang, Lin
Wang, Qi
Huang, Qinghua
Mo, Dan
author_sort Li, Meiqin
collection PubMed
description Background: Natural killer (NK) cells are crucial components of the innate immune system that fight tumors and viral infections. Patients with colorectal cancer (CRC) have a poor prognosis, and immunotherapeutic tools play a key role in the treatment of CRC. Methods: Public data on CRC patients was collected from the TCGA and the GEO databases. Tissue data of CRC patients were collected from Guangxi Medical University Affiliated Cancer Hospital. An NK-related prognostic model was developed by the least absolute shrinkage and selection operator (LASSO) and Cox regression method. Validation data were collected from different clinical subgroups and an external independent validation cohort to verify the model’s accuracy. In addition, multiple external independent immunotherapy datasets were collected to further examine the value of NK-related risk scores (NKRS) in the prediction of immunotherapy response. Potential biological functions of key genes were examined by methods of cell proliferation, apoptosis and Western blotting. Results: A novel prognostic model for CRC patients based on NK-related genes was developed and NKRS was generated. There was a significantly poorer prognosis among the high-NKRS group. Based on immune response prediction, patients with low NKRS may be more suitable for immunotherapy and they are more sensitive to immunotherapy. The proliferation rate of CRC cells was significantly reduced and apoptosis of CRC cells was increased after SLC2A3 was knocked down. SLC2A3 was also found to be associated with the TGF-β signaling pathway. Conclusion: NKRS has potential applications for predicting prognostic status and response to immunotherapy in CRC patients. SLC2A3 has potential as a therapeutic target for CRC.
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spelling pubmed-106794162023-11-13 Natural killer cell-related prognosis signature predicts immune response in colon cancer patients Li, Meiqin Song, Jingqing Wang, Lin Wang, Qi Huang, Qinghua Mo, Dan Front Pharmacol Pharmacology Background: Natural killer (NK) cells are crucial components of the innate immune system that fight tumors and viral infections. Patients with colorectal cancer (CRC) have a poor prognosis, and immunotherapeutic tools play a key role in the treatment of CRC. Methods: Public data on CRC patients was collected from the TCGA and the GEO databases. Tissue data of CRC patients were collected from Guangxi Medical University Affiliated Cancer Hospital. An NK-related prognostic model was developed by the least absolute shrinkage and selection operator (LASSO) and Cox regression method. Validation data were collected from different clinical subgroups and an external independent validation cohort to verify the model’s accuracy. In addition, multiple external independent immunotherapy datasets were collected to further examine the value of NK-related risk scores (NKRS) in the prediction of immunotherapy response. Potential biological functions of key genes were examined by methods of cell proliferation, apoptosis and Western blotting. Results: A novel prognostic model for CRC patients based on NK-related genes was developed and NKRS was generated. There was a significantly poorer prognosis among the high-NKRS group. Based on immune response prediction, patients with low NKRS may be more suitable for immunotherapy and they are more sensitive to immunotherapy. The proliferation rate of CRC cells was significantly reduced and apoptosis of CRC cells was increased after SLC2A3 was knocked down. SLC2A3 was also found to be associated with the TGF-β signaling pathway. Conclusion: NKRS has potential applications for predicting prognostic status and response to immunotherapy in CRC patients. SLC2A3 has potential as a therapeutic target for CRC. Frontiers Media S.A. 2023-11-13 /pmc/articles/PMC10679416/ /pubmed/38026928 http://dx.doi.org/10.3389/fphar.2023.1253169 Text en Copyright © 2023 Li, Song, Wang, Wang, Huang and Mo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Li, Meiqin
Song, Jingqing
Wang, Lin
Wang, Qi
Huang, Qinghua
Mo, Dan
Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title_full Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title_fullStr Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title_full_unstemmed Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title_short Natural killer cell-related prognosis signature predicts immune response in colon cancer patients
title_sort natural killer cell-related prognosis signature predicts immune response in colon cancer patients
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679416/
https://www.ncbi.nlm.nih.gov/pubmed/38026928
http://dx.doi.org/10.3389/fphar.2023.1253169
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