Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis

Here, we present a validated workflow to isolate sufficient viable single ovary cells from a single mouse without the need to pool from several mice. We provide steps essential for estrous staging, ovary harvesting and dissociation, ovary cell staining, data collection, and analysis. Our approach al...

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Detalles Bibliográficos
Autores principales: Bafor, Enitome E., Martin, Toni, Carrell, Jeffrey, Karwan, Megan, Kimmel, Adrienne E., Omogiade, Uyi.G., Sanford, Michael, Young, Howard A., Valencia, Julio C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679862/
https://www.ncbi.nlm.nih.gov/pubmed/37963022
http://dx.doi.org/10.1016/j.xpro.2023.102710

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679862/
https://www.ncbi.nlm.nih.gov/pubmed/37963022
http://dx.doi.org/10.1016/j.xpro.2023.102710

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