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Baited reconstruction with 2D template matching for high-resolution structure determination in vitro and in vivo without template bias

Previously we showed that 2D template matching (2DTM) can be used to localize macromolecular complexes in images recorded by cryogenic electron microscopy (cryo-EM) with high precision, even in the presence of noise and cellular background (Lucas et al., 2021; Lucas et al., 2022). Here, we show that...

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Detalles Bibliográficos
Autores principales: Lucas, Bronwyn A, Himes, Benjamin A, Grigorieff, Nikolaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681363/
https://www.ncbi.nlm.nih.gov/pubmed/38010355
http://dx.doi.org/10.7554/eLife.90486