Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylation

Large quantities of developmentally synchronized pupal intestines are required for biochemistry experiments. Here, we present a protocol for the mass isolation of staged pupal intestines during Drosophila melanogaster development based on buoyancy in sucrose for biochemical evaluation of protein ubi...

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Detalles Bibliográficos
Autores principales: Wang, Ruoxi, Velentzas, Panagiotis D., Baehrecke, Eric H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682243/
https://www.ncbi.nlm.nih.gov/pubmed/37950865
http://dx.doi.org/10.1016/j.xpro.2023.102713

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682243/
https://www.ncbi.nlm.nih.gov/pubmed/37950865
http://dx.doi.org/10.1016/j.xpro.2023.102713

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