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Mass isolation of staged Drosophila pupal intestines for analysis of protein ubiquitylation
Large quantities of developmentally synchronized pupal intestines are required for biochemistry experiments. Here, we present a protocol for the mass isolation of staged pupal intestines during Drosophila melanogaster development based on buoyancy in sucrose for biochemical evaluation of protein ubi...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682243/ https://www.ncbi.nlm.nih.gov/pubmed/37950865 http://dx.doi.org/10.1016/j.xpro.2023.102713 |