Cargando…

Optimized RT-qPCR and a novel normalization method for validating circulating miRNA biomarkers in ageing-related diseases

Circulating miRNAs have potential as minimally invasive biomarkers for diagnosing various diseases, including ageing-related disorders such as Alzheimer’s disease (AD). However, the lack of standardization in the common analysis method, RT-qPCR, and specifically in the normalization step, has result...

Descripción completa

Detalles Bibliográficos
Autores principales:	Want, Andrew, Staniak, Karolina, Grabowska-Pyrzewicz, Wioleta, Fesiuk, Aleksandra, Barczak, Anna, Gabryelewicz, Tomasz, Kulczyńska-Przybik, Agnieszka, Mroczko, Barbara, Wojda, Urszula
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682428/ https://www.ncbi.nlm.nih.gov/pubmed/38012217 http://dx.doi.org/10.1038/s41598-023-47971-3

Ejemplares similares

Antisense oligonucleotides for Alzheimer's disease therapy: from the mRNA to miRNA paradigm
por: Grabowska-Pyrzewicz, Wioleta, et al.
Publicado: (2021)

Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
por: Fauth, Maria, et al.
Publicado: (2019)

Novel Dicarboximide BK124.1 Breaks Multidrug Resistance and Shows Anticancer Efficacy in Chronic Myeloid Leukemia Preclinical Models and Patients’ CD34(+)/CD38(−) Leukemia Stem Cells
por: Stukan, Iga, et al.
Publicado: (2022)

Candidate Alzheimer’s Disease Biomarker miR-483-5p Lowers TAU Phosphorylation by Direct ERK1/2 Repression
por: Nagaraj, Siranjeevi, et al.
Publicado: (2021)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia
por: Drobna, Monika, et al.
Publicado: (2018)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

A systemic approach to screening high-throughput RT-qPCR data for a suitable set of reference circulating miRNAs
por: Pagacz, Konrad, et al.
Publicado: (2020)

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

The Implication of Reticulons (RTNs) in Neurodegenerative Diseases: From Molecular Mechanisms to Potential Diagnostic and Therapeutic Approaches
por: Kulczyńska-Przybik, Agnieszka, et al.
Publicado: (2021)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Neurogranin and VILIP-1 as Molecular Indicators of Neurodegeneration in Alzheimer’s Disease: A Systematic Review and Meta-Analysis
por: Dulewicz, Maciej, et al.
Publicado: (2020)

Inflammation in the CNS: Understanding Various Aspects of the Pathogenesis of Alzheimer's Disease
por: Doroszkiewicz, Julia, et al.
Publicado: (2022)

Evaluation of extraction kits and RT-qPCR systems adapted to high-throughput platform for circulating miRNAs
por: Tan, Geok Wee, et al.
Publicado: (2015)

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
por: Stobnicka-Kupiec, Agata, et al.
Publicado: (2022)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Custom RT-qPCR-array for glaucoma filtering surgery prognosis
por: Rodriguez-Agirretxe, Iñaki, et al.
Publicado: (2017)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Predictive factors of COVID-19 in patients with negative RT-qPCR
por: López de la Iglesia, J., et al.
Publicado: (2020)

Large-Scale RT-qPCR Diagnostics for Seed Potato Certification
por: Schumpp, Olivier, et al.
Publicado: (2021)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

miRNA length variation during macrophage stimulation confounds the interpretation of results: implications for miRNA quantification by RT-qPCR
por: Pillman, Katherine A., et al.
Publicado: (2019)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Circumventing qPCR inhibition to amplify miRNAs in plasma
por: Plieskatt, Jordan L, et al.
Publicado: (2014)

Abnormalities of DYRK1A-Cytoskeleton Complexes in the Blood Cells as Potential Biomarkers of Alzheimer’s Disease
por: Dowjat, Karol, et al.
Publicado: (2019)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
por: Shrestha, Kiran, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_d8t8g8s4tvdb7bmck9betnhb27