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Protocol for in vitro establishment of heterogeneous stem-like cultures derived from whole human glioblastoma tumors

Cultures enriched in glioblastoma stem-like cells (GSCs) are prominent in vitro models to investigate molecular determinants and therapeutic targets of glioblastoma; however, conventional GSC derivation protocols fail to preserve GSC heterogeneity. Here, we present a protocol for the propagation of...

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Detalles Bibliográficos
Autores principales: De Bacco, Francesca, Orzan, Francesca, Casanova, Elena, Prelli, Marta, Boccaccio, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684815/
https://www.ncbi.nlm.nih.gov/pubmed/37971942
http://dx.doi.org/10.1016/j.xpro.2023.102705