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Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo foll...

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Detalles Bibliográficos
Autores principales: Seki, Shinsuke, Kawabe, Toshiaki, Yamazaki, Wataru, Matsumura, Kazuaki, Oikawa, Takanori, Obata, Takahiro, Higashiya, Misako, Yano, Megumi, Eto, Tomoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684866/
https://www.ncbi.nlm.nih.gov/pubmed/38017006
http://dx.doi.org/10.1038/s41598-023-47394-0