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Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes
Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo foll...
Autores principales: | Seki, Shinsuke, Kawabe, Toshiaki, Yamazaki, Wataru, Matsumura, Kazuaki, Oikawa, Takanori, Obata, Takahiro, Higashiya, Misako, Yano, Megumi, Eto, Tomoo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684866/ https://www.ncbi.nlm.nih.gov/pubmed/38017006 http://dx.doi.org/10.1038/s41598-023-47394-0 |
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