Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates

Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases. Here, we present a protocol to pull down fluorescently tagged proteins to characterize their basal oligomeric states. We describe steps for transfecti...

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Detalles Bibliográficos
Autores principales: Djaja, Nathalie, Myong, Sua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684877/
https://www.ncbi.nlm.nih.gov/pubmed/37967012
http://dx.doi.org/10.1016/j.xpro.2023.102716

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684877/
https://www.ncbi.nlm.nih.gov/pubmed/37967012
http://dx.doi.org/10.1016/j.xpro.2023.102716

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