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Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases. Here, we present a protocol to pull down fluorescently tagged proteins to characterize their basal oligomeric states. We describe steps for transfecti...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684877/ https://www.ncbi.nlm.nih.gov/pubmed/37967012 http://dx.doi.org/10.1016/j.xpro.2023.102716 |