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Enhanced production of a recombinant xylanase (XT6): optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor

The endoxylanase XT6 produced by Geobacillus stearothermophilus is a desirable candidate for industrial applications. In this study, the gene encoding XT6 was cloned using the pET-28a expression vector and expressed in Escherichia coli BL21 (DE3) cells. Recombinant XT6 production was improved by opt...

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Detalles Bibliográficos
Autores principales: Dhaver, Priyashini, Sithole, Tariro, Pletschke, Brett, Sithole, Bruce, Govinden, Roshini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684889/
https://www.ncbi.nlm.nih.gov/pubmed/38017111
http://dx.doi.org/10.1038/s41598-023-48202-5