Cargando…

Enhanced production of a recombinant xylanase (XT6): optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor

The endoxylanase XT6 produced by Geobacillus stearothermophilus is a desirable candidate for industrial applications. In this study, the gene encoding XT6 was cloned using the pET-28a expression vector and expressed in Escherichia coli BL21 (DE3) cells. Recombinant XT6 production was improved by opt...

Descripción completa

Detalles Bibliográficos
Autores principales:	Dhaver, Priyashini, Sithole, Tariro, Pletschke, Brett, Sithole, Bruce, Govinden, Roshini
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684889/ https://www.ncbi.nlm.nih.gov/pubmed/38017111 http://dx.doi.org/10.1038/s41598-023-48202-5

Ejemplares similares

Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa
por: Dhaver, Priyashini, et al.
Publicado: (2022)

Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy
por: Dhaver, Priyashini, et al.
Publicado: (2022)

Optimization of Lipopeptide Biosurfactant Production by Salibacterium sp. 4CTb in Batch Stirred-Tank Bioreactors
por: Márquez-Villa, José Martín, et al.
Publicado: (2022)

Enhanced Natamycin production by Streptomyces natalensis in shake-flasks and stirred tank bioreactor under batch and fed-batch conditions
por: Elsayed, Elsayed Ahmed, et al.
Publicado: (2019)

A glucose tolerant β-glucosidase from a newly isolated Neofusicoccum parvum strain F7: production, purification, and characterization
por: Singh, Nivisti, et al.
Publicado: (2023)

Mycoprotein Production by Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate
por: Bakratsas, Georgios, et al.
Publicado: (2023)

Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites
por: Papaspyridi, Lefki-Maria, et al.
Publicado: (2012)

Production of bioherbicide by Phoma sp. in a stirred-tank bioreactor
por: Brun, Thiarles, et al.
Publicado: (2016)

Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation
por: Singh, Nivisti, et al.
Publicado: (2022)

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis
por: Singh, Nivisti, et al.
Publicado: (2023)

Comparison of Syngas-Fermenting Clostridia in Stirred-Tank Bioreactors and the Effects of Varying Syngas Impurities
por: Oliveira, Luis, et al.
Publicado: (2022)

Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial
por: de Oliveira, Fernanda, et al.
Publicado: (2020)

Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
por: Abdella, Asmaa, et al.
Publicado: (2020)

Production of secondary metabolites in stirred tank bioreactor co-cultures of Streptomyces noursei and Aspergillus terreus
por: Boruta, Tomasz, et al.
Publicado: (2022)

Transition from static culture to stirred tank bioreactor for the allogeneic production of therapeutic discogenic cell spheres
por: Rodriguez-Granrose, Daniel, et al.
Publicado: (2021)

Heterotrophic Cultivation of Euglena gracilis in Stirred Tank Bioreactor: A Promising Bioprocess for Sustainable Paramylon Production
por: Ivušić, Franjo, et al.
Publicado: (2022)

Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors
por: Chaverra-Muñoz, Lillibeth, et al.
Publicado: (2022)

Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
por: Machado, Suellen, et al.
Publicado: (2022)

Evaluation of a disposable stirred tank bioreactor for cultivation of mammalian cells
por: Hähnel, Alexander, et al.
Publicado: (2011)

Optimisation of Cellulase Production by Penicillium funiculosum in a Stirred Tank Bioreactor Using Multivariate Response Surface Analysis
por: de Albuquerque de Carvalho, Marcelle Lins, et al.
Publicado: (2014)

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor
por: Kokotkiewicz, Adam, et al.
Publicado: (2013)

Characterization and performance of the Allegro™ STR 200 single-use stirred tank bioreactor
por: Rees, Byron, et al.
Publicado: (2015)

A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor
por: Martiniano, Sabrina Evelin, et al.
Publicado: (2020)

Characterization and performance of the Allegro™ STR 1000 single-use stirred tank bioreactor system
por: Irfan, Kerem, et al.
Publicado: (2015)

Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated Manufacturing
por: Gatla, Himavanth, et al.
Publicado: (2022)

Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor
por: Arifin, Mohd Azmir, et al.
Publicado: (2010)

Biodegradation of high concentrations of benzene vapors in a two phase partition stirred tank bioreactor
por: Karimi, Ali, et al.
Publicado: (2013)

Production of pullulan by a thermotolerant aureobasidium pullulans strain in non-stirred fed batch fermentation process
por: Singh, Ranjan, et al.
Publicado: (2012)

Enhancement of Oxygen Mass Transfer and Gas Holdup Using Palm Oil in Stirred Tank Bioreactors with Xanthan Solutions as Simulated Viscous Fermentation Broths
por: Mohd Sauid, Suhaila, et al.
Publicado: (2013)

High Throughput Manufacturing of Bacteriophages Using Continuous Stirred Tank Bioreactors Connected in Series to Ensure Optimum Host Bacteria Physiology for Phage Production
por: Mancuso, Francesco, et al.
Publicado: (2018)

Comparisons of optically monitored small-scale stirred tank vessels to optically controlled disposable bag bioreactors
por: Hanson, Michael A, et al.
Publicado: (2009)

Oxygen mass transfer in a stirred tank bioreactor using different impeller configurations for environmental purposes
por: Karimi, Ali, et al.
Publicado: (2013)

Influence of shear force on ex vivo expansion of hematopoietic model cells in a stirred tank bioreactor
por: Mohseni, Sofia, et al.
Publicado: (2023)

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
por: Hemmerich, Johannes, et al.
Publicado: (2014)

Dynamic Modeling of the Impact of Temperature Changes on CO(2) Production during Milk Fermentation in Batch Bioreactors
por: Ritonja, Jožef, et al.
Publicado: (2021)

Characterization of the Metabolic Response of Streptomyces clavuligerus to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production
por: Gómez-Ríos, David, et al.
Publicado: (2019)

Production of Calcaride A by Calcarisporium sp. in Shaken Flasks and Stirred Bioreactors
por: Tamminen, Anu, et al.
Publicado: (2015)

The productivity limit of manufacturing blood cell therapy in scalable stirred bioreactors
por: Bayley, Rachel, et al.
Publicado: (2017)

Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
por: Eicke, Dorothee, et al.
Publicado: (2018)

Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation
por: Shang, Tingting, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_pot7pooh0qhm8hb8pe8kro32nu