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Evaluating the stability of host-reference gene expression and simultaneously quantifying parasite burden and host immune responses in murine malaria
The efficacy of pre-erythrocytic stage malaria antigens or vaccine platforms is routinely assessed in murine models challenged with Plasmodium sporozoites. Relative liver-stage parasite burden is quantified using reverse transcription quantitative PCR (RTqPCR), which relies on constitutively express...
Autores principales: | Browne, Daniel J., Kelly, Ashton M., Brady, Jamie, Proietti, Carla, Sarathkumara, Yomani D., Pattinson, David J., Doolan, Denise L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687243/ https://www.ncbi.nlm.nih.gov/pubmed/38030676 http://dx.doi.org/10.1038/s41598-023-48066-9 |
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