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Protocol to induce de novo lipid droplets in Saccharomyces cerevisiae

The mechanisms underlying lipid droplet (LD) biogenesis in the endoplasmic reticulum are not completely understood. Here, we present a protocol for inducing de novo LDs containing either triacylglycerol (TAG) or sterol esters (SE) in Saccharomyces cerevisiae. We describe steps for generating conditi...

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Detalles Bibliográficos
Autores principales: Khatri, Amit, Choudhary, Vineet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687286/
https://www.ncbi.nlm.nih.gov/pubmed/37995191
http://dx.doi.org/10.1016/j.xpro.2023.102732