Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions

In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dua...

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Detalles Bibliográficos
Autores principales: Aoyama, Minako, Agari, Kazuko, Sun-Wada, Ge-Hong, Futai, Masamitsu, Wada, Yoh
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069132/
https://www.ncbi.nlm.nih.gov/pubmed/15784610
http://dx.doi.org/10.1093/nar/gni055

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069132/
https://www.ncbi.nlm.nih.gov/pubmed/15784610
http://dx.doi.org/10.1093/nar/gni055

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