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Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions
In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dua...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069132/ https://www.ncbi.nlm.nih.gov/pubmed/15784610 http://dx.doi.org/10.1093/nar/gni055 |