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Protocol to study human mitochondrial ribosome using quantitative density gradient analysis by mass spectrometry and complexome profiling analysis

Dynamic macromolecular complexes containing a large number of components are often difficult to study using conventional approaches, such as immunoblotting. Here, we present a protocol for the analysis of macromolecular complexes in near-native conditions using a flexible setup to suit different cel...

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Detalles Bibliográficos
Autores principales: Páleníková, Petra, Minczuk, Michal, Rebelo-Guiomar, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692952/
https://www.ncbi.nlm.nih.gov/pubmed/37976156
http://dx.doi.org/10.1016/j.xpro.2023.102605