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Protocol to study human mitochondrial ribosome using quantitative density gradient analysis by mass spectrometry and complexome profiling analysis
Dynamic macromolecular complexes containing a large number of components are often difficult to study using conventional approaches, such as immunoblotting. Here, we present a protocol for the analysis of macromolecular complexes in near-native conditions using a flexible setup to suit different cel...
Autores principales: | Páleníková, Petra, Minczuk, Michal, Rebelo-Guiomar, Pedro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692952/ https://www.ncbi.nlm.nih.gov/pubmed/37976156 http://dx.doi.org/10.1016/j.xpro.2023.102605 |
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