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Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis
BACKGROUND: Sporotrichosis caused by Sporothrix brasiliensis is a globally emerging infectious disease with limited therapeutic options. Thus, we aimed to evaluate the in vitro activity of amlodipine (AML) and lufenuron (LUF) alone and their interaction with itraconazole (ITZ), the first-choice drug...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693817/ https://www.ncbi.nlm.nih.gov/pubmed/38050607 http://dx.doi.org/10.7717/peerj.16443 |
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author | Poester, Vanice Rodrigues Hidalgo, Jéssica Estefania Dávila Jardim, Lara Severo Trápaga, Mariana Rodrigues Rabello, Vanessa Brito de Souza Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Xavier, Melissa Orzechowski |
author_facet | Poester, Vanice Rodrigues Hidalgo, Jéssica Estefania Dávila Jardim, Lara Severo Trápaga, Mariana Rodrigues Rabello, Vanessa Brito de Souza Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Xavier, Melissa Orzechowski |
author_sort | Poester, Vanice Rodrigues |
collection | PubMed |
description | BACKGROUND: Sporotrichosis caused by Sporothrix brasiliensis is a globally emerging infectious disease with limited therapeutic options. Thus, we aimed to evaluate the in vitro activity of amlodipine (AML) and lufenuron (LUF) alone and their interaction with itraconazole (ITZ), the first-choice drug against S. brasiliensis. METHODS: Twenty clinical isolates of S. brasiliensis from two hyperendemic regions were tested through a microdilution assay to evaluate the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of AML and LUF. Checkerboard assay was performed with 10 isolates for both drug interactions with ITZ. RESULTS: AML showed inhibitory and fungicidal activity against all isolates included, with MIC values ranging from 32 to 256 µg/mL, and MFC from 64 to 256 µg/mL. However, none of the S. brasiliensis isolates were inhibited by the highest soluble concentration of LUF (MIC >64 µg/mL for all strains). Synergic interaction of AML and LUF with ITZ occurred in 50% and 40% of the isolates tested, without any antagonistic effects. CONCLUSION: Both repurposing drugs evaluated in our study showed a promising in vitro activity, especially in synergy with ITZ against S. brasiliensis, warranting future in vivo investigations regarding its activity. |
format | Online Article Text |
id | pubmed-10693817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106938172023-12-04 Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis Poester, Vanice Rodrigues Hidalgo, Jéssica Estefania Dávila Jardim, Lara Severo Trápaga, Mariana Rodrigues Rabello, Vanessa Brito de Souza Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Xavier, Melissa Orzechowski PeerJ Microbiology BACKGROUND: Sporotrichosis caused by Sporothrix brasiliensis is a globally emerging infectious disease with limited therapeutic options. Thus, we aimed to evaluate the in vitro activity of amlodipine (AML) and lufenuron (LUF) alone and their interaction with itraconazole (ITZ), the first-choice drug against S. brasiliensis. METHODS: Twenty clinical isolates of S. brasiliensis from two hyperendemic regions were tested through a microdilution assay to evaluate the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of AML and LUF. Checkerboard assay was performed with 10 isolates for both drug interactions with ITZ. RESULTS: AML showed inhibitory and fungicidal activity against all isolates included, with MIC values ranging from 32 to 256 µg/mL, and MFC from 64 to 256 µg/mL. However, none of the S. brasiliensis isolates were inhibited by the highest soluble concentration of LUF (MIC >64 µg/mL for all strains). Synergic interaction of AML and LUF with ITZ occurred in 50% and 40% of the isolates tested, without any antagonistic effects. CONCLUSION: Both repurposing drugs evaluated in our study showed a promising in vitro activity, especially in synergy with ITZ against S. brasiliensis, warranting future in vivo investigations regarding its activity. PeerJ Inc. 2023-11-30 /pmc/articles/PMC10693817/ /pubmed/38050607 http://dx.doi.org/10.7717/peerj.16443 Text en ©2023 Poester et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Microbiology Poester, Vanice Rodrigues Hidalgo, Jéssica Estefania Dávila Jardim, Lara Severo Trápaga, Mariana Rodrigues Rabello, Vanessa Brito de Souza Almeida-Paes, Rodrigo Zancope-Oliveira, Rosely Maria Xavier, Melissa Orzechowski Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title | Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title_full | Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title_fullStr | Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title_full_unstemmed | Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title_short | Amlodipine and lufenuron as repurposing drugs against Sporothrix brasiliensis |
title_sort | amlodipine and lufenuron as repurposing drugs against sporothrix brasiliensis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693817/ https://www.ncbi.nlm.nih.gov/pubmed/38050607 http://dx.doi.org/10.7717/peerj.16443 |
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