In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia

Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling....

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Detalles Bibliográficos
Autores principales: Omari, Sofia A., Kosasih, Hansen J., Chung, Thomas, de Bock, Charles E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694137/
http://dx.doi.org/10.1016/j.heliyon.2023.e22085
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author Omari, Sofia A.
Kosasih, Hansen J.
Chung, Thomas
de Bock, Charles E.
author_facet Omari, Sofia A.
Kosasih, Hansen J.
Chung, Thomas
de Bock, Charles E.
author_sort Omari, Sofia A.
collection PubMed
description Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling. These are subsequently used for the viral transduction of the IL-3 cytokine-dependent Ba/F3 cell line or murine hematopoietic stem and progenitor cells (HSPCs) for in vitro and in vivo modelling of cytokine-independent growth or leukemia initiation respectively.
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spelling pubmed-106941372023-12-05 In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia Omari, Sofia A. Kosasih, Hansen J. Chung, Thomas de Bock, Charles E. Heliyon Research Article Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling. These are subsequently used for the viral transduction of the IL-3 cytokine-dependent Ba/F3 cell line or murine hematopoietic stem and progenitor cells (HSPCs) for in vitro and in vivo modelling of cytokine-independent growth or leukemia initiation respectively. Elsevier 2023-11-08 /pmc/articles/PMC10694137/ http://dx.doi.org/10.1016/j.heliyon.2023.e22085 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Omari, Sofia A.
Kosasih, Hansen J.
Chung, Thomas
de Bock, Charles E.
In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title_full In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title_fullStr In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title_full_unstemmed In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title_short In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
title_sort in vitro and in vivo modelling of mutant jak3/stat5 signaling in leukemia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694137/
http://dx.doi.org/10.1016/j.heliyon.2023.e22085
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