Protocol to characterize the melanoma tumor immune microenvironment in mice from single cell to flow cytometry analysis

Here, we present a protocol to study and describe immune cells that surround or infiltrate tumor cells or get through the body of a melanoma syngeneic mice model. We describe steps for creating and establishing the syngeneic mouse model, euthanasia, and tumor or organ harvest. We then detail procedu...

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Detalles Bibliográficos
Autores principales: Sisó, Pol, de la Rosa, Inés, Ríos, Christopher, Panosa, Anaïs, Verdaguer, Joan, Martí, RosaMaria, Macià, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694585/
https://www.ncbi.nlm.nih.gov/pubmed/37979181
http://dx.doi.org/10.1016/j.xpro.2023.102690

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694585/
https://www.ncbi.nlm.nih.gov/pubmed/37979181
http://dx.doi.org/10.1016/j.xpro.2023.102690

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