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Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica

2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering...

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Detalles Bibliográficos
Autores principales: Zhang, Yan, Zhang, Xuejing, Liu, Haiyan, Hou, Jin, Liu, Mengmeng, Qi, Qingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694633/
http://dx.doi.org/10.1016/j.synbio.2023.11.002
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author Zhang, Yan
Zhang, Xuejing
Liu, Haiyan
Hou, Jin
Liu, Mengmeng
Qi, Qingsheng
author_facet Zhang, Yan
Zhang, Xuejing
Liu, Haiyan
Hou, Jin
Liu, Mengmeng
Qi, Qingsheng
author_sort Zhang, Yan
collection PubMed
description 2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering of Y. lipolytica for efficient 2′-FL production was performed. By fusing different protein tags, the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL production. By iterative integration of the related genes, increasing the precursor supply, and promoting NADPH regeneration, the 2′-FL synthesis was further improved. The final 2′-FL titer, 41.10 g/L, was obtained in the strain F5-1. Our work reports the highest 2′-FL production in Y. lipolytica, and demonstrates that Y. lipolytica is an efficient microbial chassis for the synthesis of oligosaccharides.
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spelling pubmed-106946332023-12-05 Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica Zhang, Yan Zhang, Xuejing Liu, Haiyan Hou, Jin Liu, Mengmeng Qi, Qingsheng Synth Syst Biotechnol Original Research Article 2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering of Y. lipolytica for efficient 2′-FL production was performed. By fusing different protein tags, the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL production. By iterative integration of the related genes, increasing the precursor supply, and promoting NADPH regeneration, the 2′-FL synthesis was further improved. The final 2′-FL titer, 41.10 g/L, was obtained in the strain F5-1. Our work reports the highest 2′-FL production in Y. lipolytica, and demonstrates that Y. lipolytica is an efficient microbial chassis for the synthesis of oligosaccharides. KeAi Publishing 2023-11-15 /pmc/articles/PMC10694633/ http://dx.doi.org/10.1016/j.synbio.2023.11.002 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Zhang, Yan
Zhang, Xuejing
Liu, Haiyan
Hou, Jin
Liu, Mengmeng
Qi, Qingsheng
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title_full Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title_fullStr Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title_full_unstemmed Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title_short Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
title_sort efficient production of 2′-fucosyllactose in unconventional yeast yarrowia lipolytica
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694633/
http://dx.doi.org/10.1016/j.synbio.2023.11.002
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