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Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica
2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694633/ http://dx.doi.org/10.1016/j.synbio.2023.11.002 |
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author | Zhang, Yan Zhang, Xuejing Liu, Haiyan Hou, Jin Liu, Mengmeng Qi, Qingsheng |
author_facet | Zhang, Yan Zhang, Xuejing Liu, Haiyan Hou, Jin Liu, Mengmeng Qi, Qingsheng |
author_sort | Zhang, Yan |
collection | PubMed |
description | 2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering of Y. lipolytica for efficient 2′-FL production was performed. By fusing different protein tags, the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL production. By iterative integration of the related genes, increasing the precursor supply, and promoting NADPH regeneration, the 2′-FL synthesis was further improved. The final 2′-FL titer, 41.10 g/L, was obtained in the strain F5-1. Our work reports the highest 2′-FL production in Y. lipolytica, and demonstrates that Y. lipolytica is an efficient microbial chassis for the synthesis of oligosaccharides. |
format | Online Article Text |
id | pubmed-10694633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-106946332023-12-05 Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica Zhang, Yan Zhang, Xuejing Liu, Haiyan Hou, Jin Liu, Mengmeng Qi, Qingsheng Synth Syst Biotechnol Original Research Article 2′-Fucosyllactose (2′-FL) has great application value as a nutritional component and the whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia lipolytica has great potential in oligosaccharide synthesis and large-scale fermentation. In this study, systematic engineering of Y. lipolytica for efficient 2′-FL production was performed. By fusing different protein tags, the synthesis of 2′-FL was optimized and the ubiquitin tag was demonstrated to be the best choice to increase the 2′-FL production. By iterative integration of the related genes, increasing the precursor supply, and promoting NADPH regeneration, the 2′-FL synthesis was further improved. The final 2′-FL titer, 41.10 g/L, was obtained in the strain F5-1. Our work reports the highest 2′-FL production in Y. lipolytica, and demonstrates that Y. lipolytica is an efficient microbial chassis for the synthesis of oligosaccharides. KeAi Publishing 2023-11-15 /pmc/articles/PMC10694633/ http://dx.doi.org/10.1016/j.synbio.2023.11.002 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Article Zhang, Yan Zhang, Xuejing Liu, Haiyan Hou, Jin Liu, Mengmeng Qi, Qingsheng Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title_full | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title_fullStr | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title_full_unstemmed | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title_short | Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica |
title_sort | efficient production of 2′-fucosyllactose in unconventional yeast yarrowia lipolytica |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694633/ http://dx.doi.org/10.1016/j.synbio.2023.11.002 |
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