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Estimation of the number of single-photon emitters for multiple fluorophores with the same spectral signature
Fluorescence microscopy is of vital importance for understanding biological function. However, most fluorescence experiments are only qualitative inasmuch as the absolute number of fluorescent particles can often not be determined. Additionally, conventional approaches to measuring fluorescence inte...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Vacuum Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694824/ http://dx.doi.org/10.1116/5.0162501 |