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Estimation of the number of single-photon emitters for multiple fluorophores with the same spectral signature

Fluorescence microscopy is of vital importance for understanding biological function. However, most fluorescence experiments are only qualitative inasmuch as the absolute number of fluorescent particles can often not be determined. Additionally, conventional approaches to measuring fluorescence inte...

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Detalles Bibliográficos
Autores principales: Li, Wenchao, Li, Shuo, Brown, Timothy C., Sun, Qiang, Wang, Xuezhi, Yakovlev, Vladislav V., Kealy, Allison, Moran, Bill, Greentree, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Vacuum Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694824/
http://dx.doi.org/10.1116/5.0162501