Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system

OBJECTIVE: The purpose of this study is to reduce the spread of the vanA gene by curing the vanA-harboring plasmid of vancomycin-resistant using the CRISPR-Cas9 system. METHODS: Two specific spacer sequence (sgRNAs) specific was designed to target the vanA gene and cloned into plasmid CRISPR-Cas9. T...

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Detalles Bibliográficos
Autores principales: Tao, Shuan, Hu, Chunwei, Fang, Yewei, Zhang, He, Xu, Yao, Zheng, Lin, Chen, Luyan, Liang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694887/
http://dx.doi.org/10.1186/s12866-023-03136-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694887/
http://dx.doi.org/10.1186/s12866-023-03136-w

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