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Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway
Spinal cord injury (SCI) causes severe axon damage, usually leading to permanent paraparesis, which still lacks effective regenerative therapy. Recent studies have suggested that exosomes derived from neural stem cells (NSCs) may hold promise as attractive candidates for SCI treatment. Epidermal Gro...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696309/ http://dx.doi.org/10.1016/j.bioactmat.2023.11.013 |
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author | Qin, Tian Li, Chengjun Xu, Yan Qin, Yiming Jin, Yuxin He, Rundong Luo, Zixiang Zhao, Jinyun Duan, Chunyue Lu, Hongbin Cao, Yong Hu, Jianzhong |
author_facet | Qin, Tian Li, Chengjun Xu, Yan Qin, Yiming Jin, Yuxin He, Rundong Luo, Zixiang Zhao, Jinyun Duan, Chunyue Lu, Hongbin Cao, Yong Hu, Jianzhong |
author_sort | Qin, Tian |
collection | PubMed |
description | Spinal cord injury (SCI) causes severe axon damage, usually leading to permanent paraparesis, which still lacks effective regenerative therapy. Recent studies have suggested that exosomes derived from neural stem cells (NSCs) may hold promise as attractive candidates for SCI treatment. Epidermal Growth Factor Receptor positive NSC (EGFR(+)NSC) is a subpopulation of endogenous NSCs, showing strong regenerative capability in central nervous system disease. In the current study, we isolated exosomes from the EGFR(+)NSCs (EGFR(+)NSCs-Exos) and discovered that local delivery of EGFR(+)NSCs-Exos can effectively promote neurite regrowth in the injury site of spinal cord-injured mice and improve their neurological function recovery. Using the miRNA-seq, we firstly characterized the microRNAs (miRNAs) cargo of EGFR(+)NSCs-Exos and identified miR-34a-5p which was highly enriched in EGFR(+)NSCs derived exosomes. We further interpreted that exosomal miR-34a-5p could be transferred to neurons and inhibit the HDAC6 expression by directly binding to its mRNA, contributing to microtubule stabilization and autophagy induction for aiding SCI repair. Overall, our research demonstrated a novel therapeutic approach to improving neurological functional recovery by using exosomes secreted from a subpopulation of endogenous NSCs and providing a precise cell-free treatment strategy for SCI repair. |
format | Online Article Text |
id | pubmed-10696309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-106963092023-12-06 Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway Qin, Tian Li, Chengjun Xu, Yan Qin, Yiming Jin, Yuxin He, Rundong Luo, Zixiang Zhao, Jinyun Duan, Chunyue Lu, Hongbin Cao, Yong Hu, Jianzhong Bioact Mater Article Spinal cord injury (SCI) causes severe axon damage, usually leading to permanent paraparesis, which still lacks effective regenerative therapy. Recent studies have suggested that exosomes derived from neural stem cells (NSCs) may hold promise as attractive candidates for SCI treatment. Epidermal Growth Factor Receptor positive NSC (EGFR(+)NSC) is a subpopulation of endogenous NSCs, showing strong regenerative capability in central nervous system disease. In the current study, we isolated exosomes from the EGFR(+)NSCs (EGFR(+)NSCs-Exos) and discovered that local delivery of EGFR(+)NSCs-Exos can effectively promote neurite regrowth in the injury site of spinal cord-injured mice and improve their neurological function recovery. Using the miRNA-seq, we firstly characterized the microRNAs (miRNAs) cargo of EGFR(+)NSCs-Exos and identified miR-34a-5p which was highly enriched in EGFR(+)NSCs derived exosomes. We further interpreted that exosomal miR-34a-5p could be transferred to neurons and inhibit the HDAC6 expression by directly binding to its mRNA, contributing to microtubule stabilization and autophagy induction for aiding SCI repair. Overall, our research demonstrated a novel therapeutic approach to improving neurological functional recovery by using exosomes secreted from a subpopulation of endogenous NSCs and providing a precise cell-free treatment strategy for SCI repair. KeAi Publishing 2023-11-28 /pmc/articles/PMC10696309/ http://dx.doi.org/10.1016/j.bioactmat.2023.11.013 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Qin, Tian Li, Chengjun Xu, Yan Qin, Yiming Jin, Yuxin He, Rundong Luo, Zixiang Zhao, Jinyun Duan, Chunyue Lu, Hongbin Cao, Yong Hu, Jianzhong Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title | Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title_full | Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title_fullStr | Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title_full_unstemmed | Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title_short | Local delivery of EGFR(+)NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway |
title_sort | local delivery of egfr(+)nscs-derived exosomes promotes neural regeneration post spinal cord injury via mir-34a-5p/hdac6 pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696309/ http://dx.doi.org/10.1016/j.bioactmat.2023.11.013 |
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