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Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage
The success of long polynucleotide de novo synthesis is largely dependent on the quality and purity of the oligonucleotides used. Generally, the primary product of any synthesis reaction is directly cloned, and clones with correct products have to be identified. In this study, a novel strategy has b...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1072809/ https://www.ncbi.nlm.nih.gov/pubmed/15800209 http://dx.doi.org/10.1093/nar/gni058 |