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Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli
λ-Red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) g...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1072810/ https://www.ncbi.nlm.nih.gov/pubmed/15800210 http://dx.doi.org/10.1093/nar/gni059 |