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Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli

λ-Red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) g...

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Detalles Bibliográficos
Autores principales:	Wong, Queenie N. Y., Ng, Vivian C. W., Lin, Marie C. M., Kung, Hsiang-fu, Chan, Danny, Huang, Jian-Dong
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2005
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1072810/ https://www.ncbi.nlm.nih.gov/pubmed/15800210 http://dx.doi.org/10.1093/nar/gni059

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