Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP→YFP→mRFP FRET detected by flow cytometry

The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between three proteins microscopically. Flow cytometry offers excellent sensitivity, effective signal separation and the c...

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Detalles Bibliográficos
Autores principales: He, Liusheng, Wu, Xiaoli, Simone, James, Hewgill, Derek, Lipsky, Peter E.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074310/
https://www.ncbi.nlm.nih.gov/pubmed/15805120
http://dx.doi.org/10.1093/nar/gni057

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074310/
https://www.ncbi.nlm.nih.gov/pubmed/15805120
http://dx.doi.org/10.1093/nar/gni057

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