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An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi

RNA interference (RNAi) mediates gene silencing in many eukaryotes and has been widely used to investigate gene functions. A common method to induce sustained RNAi is introducing plasmids that synthesize short hairpin RNAs (shRNAs) using Pol III promoters. While these promoters synthesize shRNAs and...

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Detalles Bibliográficos
Autores principales: Zhou, Hongxia, Xia, Xu Gang, Xu, Zuoshang
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074311/
https://www.ncbi.nlm.nih.gov/pubmed/15805121
http://dx.doi.org/10.1093/nar/gni061