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tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells

When oligonucleotide probes are microinjected into cells to image the distribution of RNAs, they are rapidly sequestered into the nucleus. As a result, it is difficult to detect mRNAs in the cytoplasm of living cells. We were able to overcome this process by attaching tRNA transcripts to the probes....

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Detalles Bibliográficos
Autores principales: Mhlanga, Musa M., Vargas, Diana Y., Fung, Cindy W., Kramer, Fred Russell, Tyagi, Sanjay
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074395/
https://www.ncbi.nlm.nih.gov/pubmed/15809226
http://dx.doi.org/10.1093/nar/gki302