Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription

A method for the isolation of genomic fragments of RNA virus based on cDNA representational difference analysis (cDNA RDA) was developed. cDNA RDA has been applied for the subtraction of poly(A)(+) RNAs but not for poly(A)(−) RNAs, such as RNA virus genomes, owing to the vast quantity of ribosomal R...

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Detalles Bibliográficos
Autores principales: Endoh, Daiji, Mizutani, Tetsuya, Kirisawa, Rikio, Maki, Yoshiyuki, Saito, Hidetoshi, Kon, Yasuhiro, Morikawa, Shigeru, Hayashi, Masanobu
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074749/
https://www.ncbi.nlm.nih.gov/pubmed/15817564
http://dx.doi.org/10.1093/nar/gni064

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074749/
https://www.ncbi.nlm.nih.gov/pubmed/15817564
http://dx.doi.org/10.1093/nar/gni064

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