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Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription
A method for the isolation of genomic fragments of RNA virus based on cDNA representational difference analysis (cDNA RDA) was developed. cDNA RDA has been applied for the subtraction of poly(A)(+) RNAs but not for poly(A)(−) RNAs, such as RNA virus genomes, owing to the vast quantity of ribosomal R...
| Autores principales: | , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074749/ https://www.ncbi.nlm.nih.gov/pubmed/15817564 http://dx.doi.org/10.1093/nar/gni064 |