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How to find soluble proteins: a comprehensive analysis of alpha/beta hydrolases for recombinant expression in E. coli
BACKGROUND: In screening of libraries derived by expression cloning, expression of active proteins in E. coli can be limited by formation of inclusion bodies. In these cases it would be desirable to enrich gene libraries for coding sequences with soluble gene products in E. coli and thus to improve...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1079826/ https://www.ncbi.nlm.nih.gov/pubmed/15804363 http://dx.doi.org/10.1186/1471-2164-6-49 |