Increased DNA microarray hybridization specificity using sscDNA targets

BACKGROUND: The most widely used amplification method for microarray analysis of gene expression uses T7 RNA polymerase-driven in vitro transcription (IVT) to produce complementary RNA (cRNA) that can be hybridized to arrays. However, multiple rounds of amplification are required when assaying very...

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Detalles Bibliográficos
Autores principales: Barker, Christopher S, Griffin, Chandi, Dolganov, Gregory M, Hanspers, Kristina, Yang, Jean Yee Hwa, Erle, David J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1090574/
https://www.ncbi.nlm.nih.gov/pubmed/15847692
http://dx.doi.org/10.1186/1471-2164-6-57

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1090574/
https://www.ncbi.nlm.nih.gov/pubmed/15847692
http://dx.doi.org/10.1186/1471-2164-6-57

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