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Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong?

BACKGROUND: When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS: Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparen...

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Detalles Bibliográficos
Autores principales: Skern, Rasmus, Frost, Petter, Nilsen, Frank
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1090581/
https://www.ncbi.nlm.nih.gov/pubmed/15854230
http://dx.doi.org/10.1186/1471-2199-6-10