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A modification of Representational Difference Analysis, with application to the cloning of a candidate in the Reelin signalling pathway
BACKGROUND: cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. RESULTS: In the post-hybridization mix, the 5'-en...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC113762/ https://www.ncbi.nlm.nih.gov/pubmed/12022920 http://dx.doi.org/10.1186/1471-2199-3-6 |