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Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library
The Lactococcus lactis Ll.LtrB group II intron retrohomes by reverse-splicing into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it to prime reverse transcription of the inserted intron RNA. The protein and intron RNA function...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1148171/ https://www.ncbi.nlm.nih.gov/pubmed/15947133 http://dx.doi.org/10.1093/nar/gki649 |