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Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library

The Lactococcus lactis Ll.LtrB group II intron retrohomes by reverse-splicing into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it to prime reverse transcription of the inserted intron RNA. The protein and intron RNA function...

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Detalles Bibliográficos
Autores principales: Yao, Jun, Zhong, Jin, Lambowitz, Alan M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1148171/
https://www.ncbi.nlm.nih.gov/pubmed/15947133
http://dx.doi.org/10.1093/nar/gki649

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