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Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli

BACKGROUND: It has been shown that proteins fused to ubiquitin undergo greater expression in E. coli and are easier to purify and renaturate than nonhybrid foreign proteins. However, there is no commercial source of large quantities of specific deubiquitinating proteases. This is the reason why hybr...

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Detalles Bibliográficos
Autores principales: Wojtowicz, Anna, Mazurkiewicz-Pisarek, Anna, Plucienniczak, Grazyna, Mikiewicz-Sygula, Diana, Chojnacka, Luiza, Lukasiewicz, Natalia, Plucienniczak, Andrzej
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1156937/
https://www.ncbi.nlm.nih.gov/pubmed/15924623
http://dx.doi.org/10.1186/1475-2859-4-17