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Efficacy of SSH PCR in isolating differentially expressed genes

BACKGROUND: Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity, the method has not been thoroughly studied for its practical efficacy and potential limitations. RESULTS: To determ...

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Detalles Bibliográficos
Autores principales: Ji, Wan, Wright, Matthew B, Cai, Li, Flament, Angel, Lindpaintner, Klaus
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC115870/
https://www.ncbi.nlm.nih.gov/pubmed/12033988
http://dx.doi.org/10.1186/1471-2164-3-12