Immobilized stem–loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA

We have designed and evaluated novel DNA stem–loop structured probes for enzymatic detection of nucleic acid targets. These probes constitute a novel class of conformational switches for enzymatic activity, which in the absence of a target sterically shield an affinity label and upon hybridization o...

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Detalles Bibliográficos
Autores principales: Bockisch, Benjamin, Grunwald, Thomas, Spillner, Edzard, Bredehorst, Reinhard
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1159122/
https://www.ncbi.nlm.nih.gov/pubmed/15987786
http://dx.doi.org/10.1093/nar/gni101

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1159122/
https://www.ncbi.nlm.nih.gov/pubmed/15987786
http://dx.doi.org/10.1093/nar/gni101

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