An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants

BACKGROUND: Traditional gene replacement procedures are still time-consuming. They usually necessitate cloning of the gene to be mutated, insertional inactivation of the gene with an antibiotic resistance cassette and exchange of the plasmid-borne mutant allele with the bacterial chromosome. PCR and...

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Detalles Bibliográficos
Autores principales: Choi, Kyoung-Hee, Schweizer, Herbert P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1173109/
https://www.ncbi.nlm.nih.gov/pubmed/15907219
http://dx.doi.org/10.1186/1471-2180-5-30

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1173109/
https://www.ncbi.nlm.nih.gov/pubmed/15907219
http://dx.doi.org/10.1186/1471-2180-5-30

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