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An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants
BACKGROUND: Traditional gene replacement procedures are still time-consuming. They usually necessitate cloning of the gene to be mutated, insertional inactivation of the gene with an antibiotic resistance cassette and exchange of the plasmid-borne mutant allele with the bacterial chromosome. PCR and...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1173109/ https://www.ncbi.nlm.nih.gov/pubmed/15907219 http://dx.doi.org/10.1186/1471-2180-5-30 |